Analisis Histologi dan Scaning Elektron Mikroskopy (SEM) pada Somatik Embriogenesis Tanaman Porang (Amorphophallus muelleri B)
DOI:
10.25047/agriprima.v6i1.450Published:
Mar 31, 2022Issue:
Vol. 6 No. 1 (2022): MARCHKeywords:
Amorphophallus mulleri, Porang, Histologi, somatik embriogenesis, Scaning elektron mikroskopiArticles
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Abstract
Pembentukan Somatik Embriogenesis (SE) porang secara tidak langsung memiliki proses yang lambat, selama ini yang digunakan adalah induksi secara langsung karena dapat mempercepat penyediaan bibit tetapi pengetahuan tentang induksi SE secara tidak langsung sangat dibutuhkan untuk mengetahui tahapan perkembangannya. Kami menyajikan pengetahuan terkait interaksi hormon 2,4-D dan NAA terhadap induksi kalus, histologi dan scanning elektron microscop (SEM) kalus embriogenik tanaman porang (Amorphophallus muelleri). Tujuan penelitian ini adalah guna mengetahui interaksi pengaruh 2,4-D dan NAA dalam proses induksi SE dan mempelajari tahapan perubahan embrio serta perkembangan struktur embrio tanaman porang. Penelitian ini telah dilangsungkan selama 6 bulan sejak Mei hingga Oktober 2021. Tempat penelitian berada di Laboratorium Kultur Jaringan Agronomi Fakultas Pertanian Universitas Jember. Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) 2 Faktor dengan 3 kali ulangan. Faktor pertama ialah hormon 2,4-D dengan konsentrasi 0 ppm, 1 ppm, 2 ppm, dan faktor kedua yaitu hormon NAA dengan konsentrasi 0,5 ppm, 1 ppm, 1,5 ppm, 2 ppm, 2,5 ppm, kemudian data yang didapatkan dilakukan analisis deskriptif dan ANOVA. Hasil penelitian menunjukan perlakuan hormon 1 ppm 2,4-D dan 1,5 ppm NAA menghasilkan kalus embriogenik dan pada hasil histologi dan scaning electron mikroskopy (SEM) memperlihatkan tahapan embrio somatik porang meliputi tahapan proembrio, embrio globular, skutelar dan kolioptilar, lalu tahap koleoptilar berkembang ke tahap primodial tunas. Pada konsentrasi 1 ppm 2,4-D dan 1,5 ppm NAA terbentuk proembrio dan embrio globular yang memiliki permukaan proembrio yang halus.
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Author Biographies
Tri Handoyo, Universitas Jember
Parawita Dewanti, Universitas Jember
Kacung Hariyono, Universitas Jember
Didik Pudji Restanto, Universitas Jember
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