Transformasi Gen SoSPS1 dengan Menggunakan enhancer OsADH dan Eksplan Kalus Somatik Embriogenis pada Tanaman Tebu
DOI:
10.25047/agriprima.v6i2.477Published:
Sep 30, 2022Issue:
Vol. 6 No. 2 (2022): SEPTEMBERKeywords:
enhancer 5’OsADH, SoSPS1, Tanaman Tebu, TransformasiArticles
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Abstract
Transformasi genetik adalah metode alternatif untuk mendapatkan bibit unggul tanaman tebu dengan kandungan sukrosa tinggi. Kandungan sukrosa dalam tanaman tebu dipengaruhi oleh keberadaan enzim SPS yang dikode oleh gen SoSPS1. Enzim SPS berperan penting dalam proses pembentukan dan akumulasi sukrosa pada tanaman. Transformasi genetik SoSPS1 diharapkan dapat meningkatkan konsentrasi dan aktivitas enzim SPS pada tanaman. Konstruk plasmid untuk gen SoSPS1 ditambahkan enhancer OsADH yang berperan saat proses translasi protein. Adanya OsADH membantu proses penerjemahan asam amino ketika proses translasi sehingga kemampuan tanaman mengekspresikan gen tidak terganggu oleh cekaman abiotik. Konstrak gen target disispkan ke dalam T-plasmid Agrobacterium tumefaciens strain GV 3103 kemudian diinfeksikan pada kalus somatik embriogenik (SE) tanaman tebu varietas Buluh Lawang. Setelah proses transformasi, kalus diseleksi secara invitro menggunakan kanamisin 50 ppm. Media dasar setiap tahap menggunakan formulasi Murashige and Skoog. Calon tunas yang lolos hingga seleksi kelima kemudian diaklimatisasi dan dilakukan analisis PCR untuk mendeteksi adanya gen target. Hasil penelitian menunjukkan dengan eksplan kalus SE mampu menghasilkan tanaman transgenik dengan tingkat efisiensi transformasi sebesar 2,4% dan memiliki kemampuan regenerasi menjadi tanaman dengan organel lengkap.
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Author Biographies
Bambang Sugiharto, University of Jember
Zaiyin Rizky Ageng Maulidia, University of Jember
Center for Development of Advanced Science and Technology, University of Jember
Tri Handoyo, University of Jember
Ahdatu Uli Khikamil Maulidiya, Universitas Jember
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Copyright (c) 2022 Bambang Sugiharto, Zaiyin Rizky Ageng Maulidia, Tri Handoyo, Ahdatu Uli Khikamil Maulidiya (Author)
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