Regeneration of Suweg (Amorphophallus paeoniifolius (Dennst.) Nicolson) Grown in Various Concentrations of BAP and NAA under Light and Dark Storage Conditions

Abstract

Amorphophallus paeoniifolius or suweg is one of the Araceae families whose tubers are the potential to be developed as alternative foods that contain good nutrition and low glycemic index. However, the propagation of the suweg is constrained in the availability of seedlings so that the alternative propagation is needed, one of which is by tissue culture techniques. This study aims to obtain the optimal combination of media, types of explants, and culture conditions for in vitro propagation of suweg. This research was conducted with two experiments. The first experiment was the regeneration of shoots from petiole explants cultured on Murashige and Skoog (MS) media added with BAP (0, 1, 2, and 4 mg/l) and NAA (0 and 0,5 mg/l). The second experiment was the regeneration of shoots from petiole and callus explants in dark and light conditions. The results showed that MS media with the addition of 2 mg/l BAP and 0,5 mg/l NAA were the best media in the formation of callus, shoot, and roots from petiole explants (75%, 50%, 56,67%, respectively). Besides that, the best response was demonstrated by callus compared to petiole in developing more callus, shoot, and root in the dark or light conditions.