Analisis Histologi dan Scaning Elektron Mikroskopy (SEM) pada Somatik Embriogenesis Tanaman Porang (Amorphophallus muelleri B)

Authors

DOI:

10.25047/agriprima.v6i1.450

Issue:

Vol. 6 No. 1 (2022): MARCH

Keywords:

Amorphophallus mulleri, Porang, Histologi, somatik embriogenesis, Scaning elektron mikroskopi
Received: Dec 06, 2021
Accepted:
Published: Mar 31, 2022

Articles

How to Cite

Hariyanto, D. N., Handoyo, T., Dewanti, P., Hariyono, K., & Restanto, D. P. (2022). Analisis Histologi dan Scaning Elektron Mikroskopy (SEM) pada Somatik Embriogenesis Tanaman Porang (Amorphophallus muelleri B). Agriprima : Journal of Applied Agricultural Sciences, 6(1), 22–34. https://doi.org/10.25047/agriprima.v6i1.450

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Abstract

Pembentukan Somatik Embriogenesis (SE) porang secara tidak langsung memiliki proses yang lambat, selama ini yang digunakan adalah induksi secara langsung karena dapat mempercepat penyediaan bibit tetapi pengetahuan tentang induksi SE secara tidak langsung sangat dibutuhkan untuk mengetahui tahapan perkembangannya. Kami menyajikan pengetahuan terkait interaksi hormon 2,4-D dan NAA terhadap induksi kalus, histologi dan scanning elektron microscop (SEM) kalus embriogenik tanaman porang (Amorphophallus muelleri). Tujuan penelitian ini adalah guna mengetahui interaksi pengaruh 2,4-D dan NAA dalam proses induksi SE dan mempelajari tahapan perubahan embrio serta perkembangan struktur embrio tanaman porang. Penelitian ini telah dilangsungkan selama 6 bulan sejak Mei hingga Oktober 2021. Tempat penelitian berada di Laboratorium Kultur Jaringan Agronomi Fakultas Pertanian Universitas Jember. Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) 2 Faktor dengan 3 kali ulangan. Faktor pertama ialah hormon 2,4-D dengan konsentrasi 0 ppm, 1 ppm, 2 ppm, dan faktor kedua yaitu hormon NAA dengan konsentrasi 0,5 ppm, 1 ppm, 1,5 ppm, 2 ppm, 2,5 ppm, kemudian data yang didapatkan dilakukan analisis deskriptif  dan ANOVA. Hasil penelitian menunjukan perlakuan hormon 1 ppm 2,4-D dan 1,5 ppm NAA menghasilkan kalus embriogenik dan pada hasil histologi dan scaning electron mikroskopy (SEM) memperlihatkan tahapan embrio somatik porang meliputi tahapan proembrio, embrio globular, skutelar dan kolioptilar, lalu tahap koleoptilar berkembang ke tahap primodial tunas. Pada konsentrasi 1 ppm 2,4-D dan 1,5 ppm NAA terbentuk proembrio dan embrio globular yang memiliki permukaan proembrio yang  halus. 

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Author Biographies

Tri Handoyo, Universitas Jember

Parawita Dewanti, Universitas Jember

Kacung Hariyono, Universitas Jember

Didik Pudji Restanto, Universitas Jember

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