Identification of Sub1A Gene in Flooding Tolerant Commercially Superior of Sugar Cane Varieties

Abstract

Sugar cane can grow in a variety of environmental conditions that are including on marginal land such as flooded land. Flooded land can significantly reduce sugar cane production. This study aimed to find a flooding tolerant gene in 8 floodings tolerant of sugarcane commercially superior varieties such as PS 881, PS 862, PS 882, Kentung, PSJT 941, Bulu Lawang, PS 865, and Kidang Kencana and identify their similarities with the Sub1A gene. Finding of flooding tolerant gene in commercially superior varieties of sugar cane. It was expected that the commercially superior varieties of sugar cane could be planted in flooding land and could provide additional contributions to national sugar production. Finally, it could reduce sugar import continuously by the government every year and as the solution to increasing food production based land area in the face of climate change. The steps were setting flooding conditions inland. Then, sugar cane was conducted DNA isolation, determination of concentration and purity, DNA confirmation through electrophoresis, and identification of Sub 1 A gene on DNA sample through PCR method with Sub 1 A 203 R / F primers and positive control of GMO rice plants containing Sub 1 A gene. The results were concluded that 8 commercially superior varieties of sugarcane such as PS 881, PS 862, PS 882, Kentung, PSJT 941, Bulu Lawang, PS 865, and Kidang Kencana, all of it have gene similar to the Sub 1 A gene. PS 881 and Kentung produced the thickest DNA sequence or gene was similar to the Sub 1 A gene.