Induksi Kalus Morfogenik dan Regenerasi Tanaman Tebu (Saccharum officinarum L.) secara In Vitro

Authors

Sitti Inderiati , FNU Yanti , Eka Ria Mentari

DOI:

10.25047/agriprima.v5i1.380

Issue:

Vol. 5 No. 1 (2021): MARCH

Keywords:

Kalus, in vitro, regenerasi, tebu
Received: Aug 11, 2020
Accepted:
Published: Mar 31, 2021

Articles

How to Cite

Inderiati, S., Yanti, F., & Mentari, E. R. (2021). Induksi Kalus Morfogenik dan Regenerasi Tanaman Tebu (Saccharum officinarum L.) secara In Vitro . Agriprima : Journal of Applied Agricultural Sciences, 5(1), 61–67. https://doi.org/10.25047/agriprima.v5i1.380

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Abstract

Propagasi in vitro merupakan cara memperoleh tanaman baru yang sehat secara cepat dan massal, Percobaan in vitro dilaksanakan untuk menginduksi kalus morfogenik dan regenerasi dari varietas tebu domestik.  Bagian terdalam gulungan daun tebu digunakan sebagai eksplan dan diinkubasi pada medium MS yang dimodifikasi dan dilengkapi dengan 2,4-D pada konsentrasi 1 mg/l, 2.5 mg/l, dan 5 mg/l untuk menginduksi pembentukan kalus.  Kalus yang terbentuk di medium induksi selanjutnya dipindahkan ke medium MS yang diperkaya dengan zat pengatur tumbuh untuk inisiasi dan perbanyakan tunas.  Persentase pembentukan kalus tertinggi dicapai pada penggunaan medium yang mengandung 2.5 mg/l 2,4-D dan maksimal proliferasi kalus morfogenik diperoleh setelah subkultur ketiga.  Dua tipe kalus yang terbentuk pada medium induksi yaitu berstruktur kering, remah dan struktur halus kompak.  Jenis kalus yang morfogenik tersebut berwarna putih dan putih kekuningan serta mudah terpisah-pisah.  Hasil proliferasi tunas dari kalus tertinggi diperoleh pada medium MS dengan kombinasi hormon tumbuh 2 mg/l Kinetin + 1 mg/l IAA dan tidak ada pertumbuhan tunas pada medium yang ditambahkan zat pengatur tumbuh tunggal, yaitu Kinetin.  Dengan demikian, penelitian ini menyimpulkan bahwa hormon tumbuh jenis sitokinin yang dikombinasi dengan auksin sangat dibutuhkan untuk regenerasi kalus tebu secara in vitro.

References

Balagalla D.N., Wijesuriya, A., Ranathunge, N.P., Perera, A.M.M.S. (2019). A Protocol for In-vitro Direct Plant Regeneration from Leaf Tissues for Micropropagation of Sugarcane, Tropical Agricultural Research, 29 (1), 55-64.

Biradar S., Biradar DP., Patil VC., Patil SS., Kambar NS. (2009). In vitro Plant Regeneration using Shoot Tip Culture in Commercial Cultivar of Sugarcane. Karnataka J. Agric. Sci., 22 (1), 21-24.

De Alcantara GB., Roberson D., De Oliveira RA., Carlos J., Filho B., Daros E. (2014). Plant Regeneration and Histological Study of the Somatic Embryogenesis of Sugarcane (Saccharum spp.) Cultivars RB855156 and RB72454. Acta Scientiarum, 36 (1), 63-72.

Dewi AM., Manuhara YSW., Winarsih S., Purnobasuki H. (2017). Growth and Development of Plant Tissue Culture and Conventional Seed Source from Several Varieties Sugarcane (Saccharum officinarumL.) on Field. Bioscience Research, 14 (2), 323-330.

Dinesh P., Thirunavukkarasu P., Saraniya A.R., 2015. In Vitro Studies of Sugarcane Variety co-91017 through Micropopagation of Shoot Tip Culture. (2015). Adv Plants Agric Res., 2 (6), 263-268. Retrieved from DOI: 10.15406/apar.2015.02.00071.

Getnet, B. (2017). Review on In Vitro Propagation of Sugarcane to Advance the Value of Tissue culture. Agri Res & Tech: Open Access J,. 5 (4). Retrieved from DOI:10.19080/ARTOAJ.2017.05.555670.

Hailu, M., Chimdessa, M., Muthswamy, M., (2018). In Vitro Propagation of Selected Sugarcane (Saccharum Officinarum L.) Varieties (C 86-165 and C 86-12) Through Shoot Apical Meristem. Int J Hort Agric.,3 (1), 1-7.

Jagadeesh B., Kumar M., Shekhar M., Udhakar P. (2011). Amenability of the Sugarcane Variety 2005 t16 to Shoot Tip Culture. Journal of Sugarcane Research, 1 (2), 75-77.

Jamil S., Shahzad R., Talha G.M., Sakhawat G., ur-Rahman S., Sultana R., Iqbal M.Z. (2017). Optimization of protocol for In Vitro Regeneration of Sugarcane (Saccharum officinarum). International Journal of Agronomi. Retrieved from https://doi.org/10.1155/2017/2089381.

Kona P., Kumar, M.H., Reddy, K.H.P., Reddy, D.M., Reddy, N.P.E., Latha P., Balaji, M.S. (2019). Effect of 2, 4 D and EMS On in vitro Regeneration in Sugarcane Cultivar, Co86032 Int. J. Curr. Microbiol. App. Sci., 8 (3), 1228-1236. Retrieved from https://doi.org/10.20546/ijcmas.2019.803.145.

OO, K.T., Htwe, M.M., San, N.N. (2018). In vitro Regeneration of Sugarcane (Saccharum officinarum) Varieties GUI 11 and PMA 96/48. Journal of Scientific and Innovative Research, 7 (1), 7-11. Available online at: www.jsirjournal.com.

Redae, M.H. & Ambaye, T.G. (2018). In Vitro Propagation of Sugarcane (Saccharum officinarum L.) Variety C86-165 through Apical Meristem. Biocatalysis and Agricultural Biotechnology, 14, 228-234. Retrieved from https://doi.org/10.1016/j.bcab.2018.03.005.

Shetty A. & Rao S. (2015). Callus Induction and Organogenesis in Sugarcane (Saccharum officinarum L.) Var 93v297. International Letters of Natural Sciences, 48, 14-22. Retrieved from DOI: 10.18052/www.scipress.com/ILNS.48.14.

Sholeha, W., Sugiharto, B., Setyati D., Dewanti, P. (2015). Induction Somatic Embryogenesis Used 2,4-Dichlorophenoxyacetic Acid (2,4-D) and Kinetin in Spindle Leaf Explant Sugarcane Jurnal Ilmu Dasar, 16 (1), 17-22. Retrieved from DOI: 10.19184/jid.v16i1.1387.

Silveira, V., De Vita, A.M., Macedo, A.F., Dias, M.F.R. (2013). Morphological and Polyamine Content Changes in Embryogenic and Non-Embryogenic Callus of Sugarcane. Plant Cell Tissue Organ Cult., Retrieved from DOI:10.1007/s11240-013-0330-2.

Suhesti, S., Khumaida, N., Watimena, G.A., Syukur, M., Husni, A., Hadipoentyanti, E., Hartati, S.R.R. (2015). Induksi Kalus dan Regenerasi Dua Varietas Tebu (Saccharum officinarum L.) secara In Vitro. Jurnal Littri, 21 (2), 77-88.

Tesfa, M. & Etwi, M. (2018). In Vitro Plant Regeneration of Sugarcane (Saccharum spp.) Variety Inoculated under Different Levels of Plant Growth Regulators. J. Plant Biochem. Physiol., 6 (4). Retrieved from DOI:10.4172/2329-9029.1000227

Tiwari, S., Arya, A., Kumar, S. (2012). Standardizing Sterilization Protocol and Establishement of Callus Culture of Sugarcane for Enhance Plant Regeneration In Vitro. Research Journal of Botany, 7 (1), 1-7.

Yadav, S. & Ahmad, A. (2013). Standardization of Callus Culture Techniques for Efficient Sugarcane Micropropagation. Cibtech Journal of Bio-Protocols, 2 (2), 29-32.

Author Biographies

Sitti Inderiati, table_open_cache

Jurusan Produksi Pertanian

FNU Yanti, Politeknik Pertanian Negeri Pangkep

Eka Ria Mentari, Politeknik Pertanian Negeri Pangkep

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