Respon Perlakuan Asam Jasmonat, Asam Salisilat dan Kitosan terhadap Produksi Metabolit Sekunder pada Kalus Padi Daun Berpigmen
DOI:
10.25047/agriprima.v8i1.599Issue:
Vol. 8 No. 1 (2024): MARCHKeywords:
Elisitasi, Kultur Jaringan, Metabolit Sekunder, PadiArticles
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Abstract
Padi Black Madras (Oryza sativa L.) merupakan padi hias yang selain biasa digunakan sebagai ornamen, juga dapat digunakan sebagai sumber pharmaceutical yang bermanfaat bagi kesehatan manusia. Namun saat ini belum banyak diketahui potensialnya menjadi sumber pharmaceutical seperti metabolit sekunder. Metabolit sekunder yang dihasilkan oleh padi black madras ini dapat diambil melalui kultur jaringan dengan cara elisitasi. Penelitian ini dilakukan untuk mengetahui respon kalus padi dan produksi metabolit sekunder pada padi Black Madras terhadap pemberian asam jasmonat, asam salisilat, dan kitosan. Hasil penelitian ini menunjukkan pemberian elisitor memberikan respon pada kalus padi dan produksi metabolit sekunder padi black madras terhadap pemberian asam jasmonat, asam salisilat, dan kitosan. Varietas terbaik didapatkan pada varietas IR64 sebagai kontrol daripada Black madras dengan perlakuan dengan kandungan fenolik (0,1548 mg GAE/g) dan flavonoid (0,2830 mg QE/g) terbaik ada pada media dengan pemberian Asam Jasmonat 10ppm. Sedangkan pada antosianin dengan pemberian yang terbaik adalah Kitosan 50ppm (PC: 2,852 CV/g FW dan PP: 0,538 CV/ test tube). Varietas Black madras lebih cenderung dalam peningkatan biomassa dengan kandungan fenolik (0,1165 mg GAE/g) dan flavonoid (0,2107 mg QE/g) pada perlakuan kontrol meskipun dalam produksi antosianin cukup tinggi dengan Kitosan 50ppm (PC: 2,832 CV/g FW) dan Asam Salisilat 10ppm (PP: 0,440 CV/ test tube) lebih daripada perlakuan kontrol.
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Author Biography
Mohammad Ubaidillah, University of Jember
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