Respon Eksplan Vanili (Vanilla planifolia) dengan Stimulasi BAP dan NAA Melalui Teknik Mikropropagasi

Dyah Nuning Erawati; Usken Fisdiana; Muhammad Kadafi

doi:10.25047/agriprima.v4i2.362

The response of Vanilla Explant (Vanilla planifolia) to BAP and NAA Stimulation through Micropropagation Technique

Authors

Dyah Nuning Erawati , Usken Fisdiana , Muhammad Kadafi

DOI:

https://doi.org/10.25047/agriprima.v4i2.362

Published:

30-09-2020

Issue:

Vol. 4 No. 2 (2020): SEPTEMBER

Keywords:

BAP, Explant, NAA, Micro propagation, Vanilla

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Erawati, D. N., Fisdiana, U., & Kadafi, M. (2020). The response of Vanilla Explant (Vanilla planifolia) to BAP and NAA Stimulation through Micropropagation Technique. Agriprima : Journal of Applied Agricultural Sciences, 4(2), 146-153. https://doi.org/10.25047/agriprima.v4i2.362

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Abstract

The development of vanilla plants with conventional vegetative propagation has limitations as a parent plant cuttings. Vanilla is potentially developed by micropropagation. The ability of vanilla explants to regenerate and differentiate to form shoots and roots in vitro needs to be controlled by regulation auxins and cytokines. Interest in the study was: 1) study the response of vanilla explants with BAP stimulation; 2) study the response of vanilla explants with NAA stimulation and 3) the interaction stimulation of BAP and NAA to the response of vanilla explants by micropropagation. The experiment was conducted in Laboratory Tissue Culture Politeknik Negeri Jember using a Completely Randomized Design (CRD) factorial. Factor 1 is the stimulation of BAP at concentration levels of 0.0, 1.0, 3.0 mg/L, and the second factor is the stimulation of NAA in the concentration level of 0.0, 0.5 mg/L. The results showed that BAP stimulation of 1 mg/L resulted in the highest average number of shoots of 6.8 shoots/ explant and NAA stimulation of 0.5 mg/L had the highest average number of roots 3.0 roots / explant.

References

Abebe, Z., Mengesha, A., Teressa, A., & Tefera, W. (2009). Efficient in vitro multiplication protocol for Vanilla planifolia using nodal explants in Ethiopia. African Journal of Biotechnology, 8(24).

Ayele, Y. B., Tefera, W., & Bantte, K. (2017). Enhanced Protocol Development for in vitro Multiplication and Rooting of Vanilla (Vanilla planifolia Andr.) Clone (Van. 2/05). Biotechnology Journal International, 1–11.

Erawati, D. N., Wardati, I., Humaida, S., & Fisdiana, U. (2020). Micropropagation of Vanilla (Vanilla planifolia Andrews) with Modification of Cytokinins. IOP Conference Series: Earth and Environmental Science, 411(1), 12009.

Jadid, N., Nurhidayati, T., & Priyono, P. (2015). In Vitro Clonal Propagation of Vanilla planifolia Andrews Using Microshoot-derived Node Explants. J. Appl. Environ. Biol. Sci., 5(6), 105–110.

Lee-Espinosa, H. E., Murgu’ia-González, J., Garc’ia-Rosas, B., Córdova-Contreras, A. L., Laguna-Cerda, A., Mijangos-Cortés, J. O., … Santana-Buzzy, N. (2008). In vitro clonal propagation of vanilla (Vanilla planifolia ‘Andrews’). HortScience, 43(2), 454–458.

Lestari, E. G. (2011). Peranan zat pengatur tumbuh dalam perbanyakan tanaman melalui kultur jaringan. Jurnal AgroBiogen, 7(1), 63–68.

Loedji, H. (2019). 8 Besar Produk Pertanian Indonesia. PortoNews.

Njoroge, A. M., Gitonga, L., Mutuma, E., Mimano, L., Macharia, C., Wasilwa, L., … Mungai, A. (2005). Propagation of high quality planting materials of Vanilla (Vanilla planifolia) through tissue culture. Kenya Agricultural Research Institute (KARI), Thika National Agricultural Research Laboratories Nairobi-Kenya, 1–4.

Palama, T. L., Menard, P., Fock, I., Choi, Y. H., Bourdon, E., Govinden-Soulange, J., … Kodja, H. (2010). Shoot differentiation from protocorm callus cultures of Vanilla planifolia (Orchidaceae): proteomic and metabolic responses at early stage. BMC Plant Biology, 10(1), 82.

Pinaria, A. G., Liew, E. C. Y., & Burgess, L. W. (2010). Fusarium species associated with vanilla stem rot in Indonesia. Australasian Plant Pathology, 39(2), 176–183.

Renuga, G., & Saravana-Kumar, S. N. (2014). Induction of vanillin related compounds from nodal explants of Vanilla planifolia using BAP and Kinetin. Asian J. Plant Sci. Res, 4(1), 53–61.

Restiani, R., Semiarti, E., & Indrianto, A. (2016). Konservasi anggrek hitam (Coelogyne pandurata Lindl.) melalui mikropropagasi pada berbagai medium kultur. Prosiding Simposium Nasional Pendidikan Biologi (Symbion 2016), 27, 393–404.

Tan, B. C., Chin, C. F., & Alderson, P. (2011). Optimisation of plantlet regeneration from leaf and nodal derived callus of Vanilla planifolia Andrews. Plant Cell, Tissue and Organ Culture (PCTOC), 105(3), 457–463.

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The response of Vanilla Explant (Vanilla planifolia) to BAP and NAA Stimulation through Micropropagation Technique

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