The response of Vanilla Explant (Vanilla planifolia) to BAP and NAA Stimulation through Micropropagation Technique

Abstract

The development of vanilla plants with conventional vegetative propagation has limitations as a parent plant cuttings. Vanilla is potentially developed by micropropagation. The ability of vanilla explants to regenerate and differentiate to form shoots and roots in vitro needs to be controlled by regulation auxins and cytokines. Interest in the study was: 1) study the response of vanilla explants with BAP stimulation; 2) study the response of vanilla explants with NAA stimulation and 3) the interaction stimulation of BAP and NAA to the response of vanilla explants by micropropagation. The experiment was conducted in Laboratory Tissue Culture Politeknik Negeri Jember using a Completely Randomized Design (CRD) factorial. Factor 1 is the stimulation of BAP at concentration levels of 0.0, 1.0, 3.0 mg/L, and the second factor is the stimulation of NAA in the concentration level of 0.0, 0.5 mg/L. The results showed that BAP stimulation of 1 mg/L resulted in the highest average number of shoots of 6.8 shoots/ explant and NAA stimulation of 0.5 mg/L had the highest average number of roots 3.0 roots / explant.