Identifikasi Gen Sub1A Pada Varietas Tebu Tahan Genangan
DOI:
10.25047/agriprima.v4i2.373Published:
Sep 30, 2020Issue:
Vol. 4 No. 2 (2020): SEPTEMBERKeywords:
Genangan, Gen Sub1A, Identifikasi gen Sub1AArticles
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Abstract
Tebu dapat tumbuh pada berbagai kondisi lingkungan yang cukup beragam termasuk pada lahan marginal seperti lahan yang sering tergenang. Lahan tergenang dapat menurunkan produksi tebu cukup signifikan. Tujuan penelitian ini adalah menemukan gen toleran kebasahan yang terdapat pada 8 varietas unggul komersial tebu yang tahan genangan yaitu PS 881, PS 862, PS 882, Kentung, PSJT 941, Bulu Lawang, PS 865 dan Kidang Kencana serta mengidentifikasi kemiripannya dengan gen Sub1A. Dengan diperolehnya gen toleran kebasahan pada varietas tebu tahan genangan di harapkan varietas tebu tersebut dapat ditanam di lahan yang sering tergenang dan dapat memberikan tambahan sumbangan produksi gula secara Nasional. Sehingga pada akhirnya dapat mengurangi impor yang secara kontinu dilakukan pemerintah setiap tahun dan sebagai solusi peningkatan produksi pangan berbasis lahan daratan dalam menghadapi perubahan iklim. Tahapan untuk memperoleh dan mengidentifikasi gen toleran kebasahan atau tahan genangan adalah pengkondisian lahan tanaman tebu tergenang, isolasi DNA dari tanaman tebu yang tergenang, penentuan konsentrasi dan kemurnian DNA yang diperoleh, konfirmasi DNA yang diisolasi dengan elektroforesis dan identifikasi gen Sub 1 A pada DNA sampel metode PCR dengan primer Sub 1 A 203 R/F dan kontrol Positif DNA tanaman padi transgenik yang mengandung gen Sub 1 A. Hasil kegiatan penelitian dapat disimpukan bahwa 8 varietas unggul komersial tebu yaitu PS 881, PS 862, PS 882, Kentung, PSJT 941, Bulu Lawang, PS 865 dan Kidang Kencana semuanya mempunyai gen yang mirip dengan gen Sub 1 A. Dengan varietas PS 881 dan Kentung menghasilkan band sekuens DNA target gen Sub 1 A paling tebal.
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Author Biographies
Ayu Puspita Arum, Universitas Jember
Sholeh Avivi, Universitas Jember
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